Effects of Pulsed Electric Fields on the Activity of Enzymes in Aqueous Solution
نویسندگان
چکیده
A group of selected enzymes were subjected to continuous pulsed electric field (PEF) treatments to evaluate the inactivation effect of PEF. For a treatment time of 126 s, 51.7% and 83.8% of pepsin was inactivated at 37.0 kV/cm and 41.8 kV/cm, respectively. Enzyme activity of polyphenol oxidase decreased 38.2% when treated at 33.6 kV/cm for 126 s. Enzyme activity decreased 18.1% and 4.0% for peroxidase treated at 34.9 kV/cm and chymotrypsin treated at 34.2 kV/cm, respectively. No significant change in lysozyme activity was observed after PEF from 0 to 38 kV/cm for 126 s. Both PEF and the induced heat contributed to the observed inactivation effect, depending on the properties of enzymes and test conditions.
منابع مشابه
Design and Fabrication of Helmholtz Coils to Study the Effects of Pulsed Electromagnetic Fields on the Healing Process in Periodontitis: Preliminary Animal Results
Background: Effects of electromagnetic fields on healing have been investigated for centuries. Substantial data indicates that exposure to electromagnetic field can lead to enhanced healing in both soft and hard tissues. Helmholtz coil is a device that generates pulsed electromagnetic fields. Objective: In this study, a pair of Helmholtz coils for enhancing thehealing process in periodontitis w...
متن کاملDielectrophoretic effect of nonuniform electric fields on the protoplast cell
In recent years, dielectrophoresis based microfluidics systems have been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. In the current study the governing electric potential equations have been solved in the presence of cell for the purpose of ...
متن کاملEffect of extremely low frequency electromagnetic fields on antioxidant enzymes in valerian (Valeriana officinalis L.). Sara Farzpourmachiani1*, Ahmad Majd2, Sedigheh Arbabian2, Davoud Dorranian3 and Mehrdad Hashemi4
In this research, the effects of electromagnetic fields of various strengths (0, 1 and 2mT) have been investigated on antioxidant enzymes activity in Valerian (Valeriana officinalis L.). The dry and wet (soaked for 30 min) Valerian seeds were exposed to electromagnetic fields 30 min per day for 3 days. Each treatment and control groups had 3 replicates and 10 seeds were employed for each replic...
متن کاملThe effect of saffron aqueous extract on oxidative stress parameters and important biochemical enzymes in the testis of streptozotocin-induced diabetic rats
Introduction: Sexual dysfunction and infertility are frequently associated with diabetes in men and experimental animals. Oxidative stress and alteration in testis are responsible for complication in diabetes. Saffron has antidiabetic and antioxidant properties that improves the functions of various organs. Therefore, the aim of the present study was to investigate the effects of administra...
متن کاملEffects of Statically Electric Fields on Freezing Parameters and Microstructures of Button Mushrooms (Agaricus bisporus)
Background and Objectives: Freezing under statically electric fields is one of the novel freezing methods to improve the quality of frozen products by controlling the nucleation process. The objective of this study was to investigate effects of freezing under electrostatic fields on the freezing parameters and microstructures of frozen button mushrooms. Materials and Methods: Mushroom samples w...
متن کامل